Journal: The Journal of investigative dermatology
Article Title: Different Propionibacterium acnes Phylotypes Induce Distinct Immune Responses and Express Unique Surface and Secreted Proteomes
doi: 10.1016/j.jid.2016.06.615
Figure Lengend Snippet: A total of 2.5 × 106/ml peripheral blood mononuclear cells were cultured with 2.5 × 106/ml live Propionibacterium acnes in the exponential phase for (a) IFN-γ or the stationary phase for (b) IL-17 and (c) IL-10. ELISA was conducted on the supernatant to determine the concentration of IFN-γ, IL-17, and IL-10. The cytokine concentrations normalized to the highest value induced by any P. acnes strain for each donor and cytokine. Each donor is represented by a different symbol. p±, presence or absence of a large plasmid; RT, ribotype.
Article Snippet: Bacterial culture P. acnes strain ATCC 6919 was obtained from American Type Culture Collection (Manassas, VA).
Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay, Plasmid Preparation